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e coli jm109 cells  (TaKaRa)


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    Structured Review

    TaKaRa e coli jm109 cells
    E Coli Jm109 Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+jm109/Monoclonal+Anti-mouse+P-cadherin/pm41518045-21-13-17
    Average 95 stars, based on 345 article reviews
    e coli jm109 cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Construct:

    Article Title: From phenotype to receptor: validating physiological clustering of Escherichia coli phages through comprehensive receptor analysis.
    Article Snippet: .. The resulting constructs were initially transformed into E. coli JM109 (Takara Bio Inc., Shiga, Japan), and positive transformants were selected on LB agar with chloramphenicol (25 μg/mL). ..

    Article Title: Engineering of acyl ligase domain in non-ribosomal peptide synthetases to change fatty acid moieties of lipopeptides
    Article Snippet: .. Second, we constructed a pUC19-based plasmid (pUC19-mycU-erm-PrplGB-fenF-ituAL-mycACP-mycAD) using an In-Fusion® HD Cloning Kit (New England Biolabs, Ipswich, MA, USA) and E. coli JM109 (Takara Bio Inc., Shiga, Japan). ..

    Article Title: Engineering of acyl ligase domain in NRPS to design fatty acid moieties of lipopeptides
    Article Snippet: .. 616 Second, we constructed a pUC19-based plasmid (pUC19-mycU-erm-PrplGB-fenF-ituAL-617 mycACP-mycAD) using an In-Fusion® HD Cloning Kit (New England Biolabs, Ipswich, MA, 618 USA) and E. coli JM109 (Takara Bio Inc., Shiga, Japan). ..

    Article Title: Method for enzymatically producing maytansinol
    Article Snippet: .. Mutant enzyme library fragments were amplified by inverse PCR using primers FpnbA_A107X and RpnbA_A107X (SEQ ID NOS: 7 and 8) and pCDF-capA-bspnbA as the template, cyclized by using T4 kinase (Toyobo) and Ligation high (Toyobo), and used to transform E. coli JM109 (TAKARA), and the bacteria were incubated overnight at 37° C. All the obtained colonies were suspended in the LB liquid medium and collected, and the plasmids were extracted from them to construct a mutant enzyme library. ..

    Transformation Assay:

    Article Title: From phenotype to receptor: validating physiological clustering of Escherichia coli phages through comprehensive receptor analysis.
    Article Snippet: .. The resulting constructs were initially transformed into E. coli JM109 (Takara Bio Inc., Shiga, Japan), and positive transformants were selected on LB agar with chloramphenicol (25 μg/mL). ..

    Plasmid Preparation:

    Article Title: Engineering of acyl ligase domain in non-ribosomal peptide synthetases to change fatty acid moieties of lipopeptides
    Article Snippet: .. Second, we constructed a pUC19-based plasmid (pUC19-mycU-erm-PrplGB-fenF-ituAL-mycACP-mycAD) using an In-Fusion® HD Cloning Kit (New England Biolabs, Ipswich, MA, USA) and E. coli JM109 (Takara Bio Inc., Shiga, Japan). ..

    Article Title: Engineering of acyl ligase domain in NRPS to design fatty acid moieties of lipopeptides
    Article Snippet: .. 616 Second, we constructed a pUC19-based plasmid (pUC19-mycU-erm-PrplGB-fenF-ituAL-617 mycACP-mycAD) using an In-Fusion® HD Cloning Kit (New England Biolabs, Ipswich, MA, 618 USA) and E. coli JM109 (Takara Bio Inc., Shiga, Japan). ..

    Cloning:

    Article Title: Engineering of acyl ligase domain in non-ribosomal peptide synthetases to change fatty acid moieties of lipopeptides
    Article Snippet: .. Second, we constructed a pUC19-based plasmid (pUC19-mycU-erm-PrplGB-fenF-ituAL-mycACP-mycAD) using an In-Fusion® HD Cloning Kit (New England Biolabs, Ipswich, MA, USA) and E. coli JM109 (Takara Bio Inc., Shiga, Japan). ..

    Article Title: Engineering of acyl ligase domain in NRPS to design fatty acid moieties of lipopeptides
    Article Snippet: .. 616 Second, we constructed a pUC19-based plasmid (pUC19-mycU-erm-PrplGB-fenF-ituAL-617 mycACP-mycAD) using an In-Fusion® HD Cloning Kit (New England Biolabs, Ipswich, MA, 618 USA) and E. coli JM109 (Takara Bio Inc., Shiga, Japan). ..

    Control:

    Article Title: Biosynthesis of High Toughness Poly(3‐Hydroxypropionate)‐Based Block Copolymers With Poly(D‐2‐Hydroxybutyrate) and Poly(D‐Lactate) Segments Using Evolved Monomer Sequence‐Regulating Polyester Synthase
    Article Snippet: .. E. coli JM109 was purchased from Takara Bio Inc. (Japan) and used as the host strain. pBSP Re phaC AR pct and pBSP Re phaC AR NDFHpctalkK were used to synthesize the polymers [ ]. pBSP Re phaC AR pct contains the phaC AR gene under the control of the phb operon promoter of Cupriavidus necator ( Ralstonia eutropha ) and the propionyl CoA transferase gene from Megasphaera elsdenii [ ]. pBSP Re phaC AR NDFHpctalkK contains the phaC AR mutant gene with N149D and F314H substitutions, and the alkK gene that encodes a medium‐chain‐length 3‐hydroxyalkanoate CoA ligase from Pseudomonas putida [ ]. ..

    Article Title: Biosynthesis of High Toughness Poly(3-Hydroxypropionate)-Based Block Copolymers With Poly(D-2-Hydroxybutyrate) and Poly(D-Lactate) Segments Using Evolved Monomer Sequence-Regulating Polyester Synthase.
    Article Snippet: This study synthesized poly(3hydroxypropionate) [P(3HP)]containing polyhydroxyalkanoate (PHA) block copolymers, P(3HP)bP[2hydroxybutyrate (2HB)] and P(3HP)bP(Dlactate) (PDLA), using Escherichia coli.. The cells expressing an evolved sequenceregulating PHA synthase, PhaCARNDFH, and propionylCoA transferase were cultured with the supplementation of the corresponding monomer precursors in the medium.. The block structure of P(3HP)bPDLA was confirmed by proton nuclear magnetic resonance analysis and solvent fractionation.

    Mutagenesis:

    Article Title: Biosynthesis of High Toughness Poly(3‐Hydroxypropionate)‐Based Block Copolymers With Poly(D‐2‐Hydroxybutyrate) and Poly(D‐Lactate) Segments Using Evolved Monomer Sequence‐Regulating Polyester Synthase
    Article Snippet: .. E. coli JM109 was purchased from Takara Bio Inc. (Japan) and used as the host strain. pBSP Re phaC AR pct and pBSP Re phaC AR NDFHpctalkK were used to synthesize the polymers [ ]. pBSP Re phaC AR pct contains the phaC AR gene under the control of the phb operon promoter of Cupriavidus necator ( Ralstonia eutropha ) and the propionyl CoA transferase gene from Megasphaera elsdenii [ ]. pBSP Re phaC AR NDFHpctalkK contains the phaC AR mutant gene with N149D and F314H substitutions, and the alkK gene that encodes a medium‐chain‐length 3‐hydroxyalkanoate CoA ligase from Pseudomonas putida [ ]. ..

    Article Title: Biosynthesis of High Toughness Poly(3-Hydroxypropionate)-Based Block Copolymers With Poly(D-2-Hydroxybutyrate) and Poly(D-Lactate) Segments Using Evolved Monomer Sequence-Regulating Polyester Synthase.
    Article Snippet: This study synthesized poly(3hydroxypropionate) [P(3HP)]containing polyhydroxyalkanoate (PHA) block copolymers, P(3HP)bP[2hydroxybutyrate (2HB)] and P(3HP)bP(Dlactate) (PDLA), using Escherichia coli.. The cells expressing an evolved sequenceregulating PHA synthase, PhaCARNDFH, and propionylCoA transferase were cultured with the supplementation of the corresponding monomer precursors in the medium.. The block structure of P(3HP)bPDLA was confirmed by proton nuclear magnetic resonance analysis and solvent fractionation.

    Article Title: Method for enzymatically producing maytansinol
    Article Snippet: .. Mutant enzyme library fragments were amplified by inverse PCR using primers FpnbA_A107X and RpnbA_A107X (SEQ ID NOS: 7 and 8) and pCDF-capA-bspnbA as the template, cyclized by using T4 kinase (Toyobo) and Ligation high (Toyobo), and used to transform E. coli JM109 (TAKARA), and the bacteria were incubated overnight at 37° C. All the obtained colonies were suspended in the LB liquid medium and collected, and the plasmids were extracted from them to construct a mutant enzyme library. ..

    Amplification:

    Article Title: Method for enzymatically producing maytansinol
    Article Snippet: .. Mutant enzyme library fragments were amplified by inverse PCR using primers FpnbA_A107X and RpnbA_A107X (SEQ ID NOS: 7 and 8) and pCDF-capA-bspnbA as the template, cyclized by using T4 kinase (Toyobo) and Ligation high (Toyobo), and used to transform E. coli JM109 (TAKARA), and the bacteria were incubated overnight at 37° C. All the obtained colonies were suspended in the LB liquid medium and collected, and the plasmids were extracted from them to construct a mutant enzyme library. ..

    Inverse PCR:

    Article Title: Method for enzymatically producing maytansinol
    Article Snippet: .. Mutant enzyme library fragments were amplified by inverse PCR using primers FpnbA_A107X and RpnbA_A107X (SEQ ID NOS: 7 and 8) and pCDF-capA-bspnbA as the template, cyclized by using T4 kinase (Toyobo) and Ligation high (Toyobo), and used to transform E. coli JM109 (TAKARA), and the bacteria were incubated overnight at 37° C. All the obtained colonies were suspended in the LB liquid medium and collected, and the plasmids were extracted from them to construct a mutant enzyme library. ..

    Ligation:

    Article Title: Method for enzymatically producing maytansinol
    Article Snippet: .. Mutant enzyme library fragments were amplified by inverse PCR using primers FpnbA_A107X and RpnbA_A107X (SEQ ID NOS: 7 and 8) and pCDF-capA-bspnbA as the template, cyclized by using T4 kinase (Toyobo) and Ligation high (Toyobo), and used to transform E. coli JM109 (TAKARA), and the bacteria were incubated overnight at 37° C. All the obtained colonies were suspended in the LB liquid medium and collected, and the plasmids were extracted from them to construct a mutant enzyme library. ..

    Article Title: Optogenetic control of Corynebacterium glutamicum gene expression.
    Article Snippet: .. The 10 μL ligation system was used to transform competent cells of E. coli JM109 (refer to the Takara E. coli competent kit instructions for cell preparation). .. Transformants with correct colony PCR results were selected and sent to Suzhou Genewiz Biotechnology Co., Ltd. for sequencing verification, resulting in the recombinant plasmid pJYW-4-GNA1-yqaB-nodC.

    Bacteria:

    Article Title: Method for enzymatically producing maytansinol
    Article Snippet: .. Mutant enzyme library fragments were amplified by inverse PCR using primers FpnbA_A107X and RpnbA_A107X (SEQ ID NOS: 7 and 8) and pCDF-capA-bspnbA as the template, cyclized by using T4 kinase (Toyobo) and Ligation high (Toyobo), and used to transform E. coli JM109 (TAKARA), and the bacteria were incubated overnight at 37° C. All the obtained colonies were suspended in the LB liquid medium and collected, and the plasmids were extracted from them to construct a mutant enzyme library. ..

    Incubation:

    Article Title: Method for enzymatically producing maytansinol
    Article Snippet: .. Mutant enzyme library fragments were amplified by inverse PCR using primers FpnbA_A107X and RpnbA_A107X (SEQ ID NOS: 7 and 8) and pCDF-capA-bspnbA as the template, cyclized by using T4 kinase (Toyobo) and Ligation high (Toyobo), and used to transform E. coli JM109 (TAKARA), and the bacteria were incubated overnight at 37° C. All the obtained colonies were suspended in the LB liquid medium and collected, and the plasmids were extracted from them to construct a mutant enzyme library. ..



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    ATCC potent antimicrobial activity against e coli jm109
    ROS generation assessment using the fluorescent probe H2DCFDA after incubation of (a) bacteria ( S. aureus , <t>E.</t> <t>coli</t> , and P. aeruginosa ) and (b) human keratinocytes and fibroblasts with TeLigNPs. Results are reported as mean values ± SD ( n = 3).
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    Image Search Results


    ROS generation assessment using the fluorescent probe H2DCFDA after incubation of (a) bacteria ( S. aureus , E. coli , and P. aeruginosa ) and (b) human keratinocytes and fibroblasts with TeLigNPs. Results are reported as mean values ± SD ( n = 3).

    Journal: RSC Advances

    Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

    doi: 10.1039/d5ra02635k

    Figure Lengend Snippet: ROS generation assessment using the fluorescent probe H2DCFDA after incubation of (a) bacteria ( S. aureus , E. coli , and P. aeruginosa ) and (b) human keratinocytes and fibroblasts with TeLigNPs. Results are reported as mean values ± SD ( n = 3).

    Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

    Techniques: Incubation, Bacteria

    Assessment of E. coli BW25113 membrane integrity following treatment with BioTe or tellurite. (a–c) Representative SEM images of untreated cells (a), BioTe-treated cells (b), and tellurite-treated cells (c). Red arrows indicate membrane perforations in treated samples. Scale bars: 1 μm. (d–f) PI staining highlights membrane-compromised cells in the untreated control (d), BioTe-treated (e), and tellurite-treated (f) groups. (g–i) SYTO 9 staining shows all cells in untreated (g), BioTe-treated (h), and tellurite-treated (i) samples. (j–l) Bright-field images of the corresponding samples. Scale bars for fluorescence and bright-field images: 10 μm. (m) β-Galactosidase activity in culture supernatants of E. coli BL21(DE3) overexpressing lacZ , treated with BioTe (3× MIC), tellurite (3× MIC), or left untreated for 1 hour. Samples were analyzed via SEM, fluorescence staining, and β-galactosidase activity measurement. Data represent mean ± SD ( n = 3). Asterisks indicate statistically significant differences ( p < 0.05).

    Journal: RSC Advances

    Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

    doi: 10.1039/d5ra02635k

    Figure Lengend Snippet: Assessment of E. coli BW25113 membrane integrity following treatment with BioTe or tellurite. (a–c) Representative SEM images of untreated cells (a), BioTe-treated cells (b), and tellurite-treated cells (c). Red arrows indicate membrane perforations in treated samples. Scale bars: 1 μm. (d–f) PI staining highlights membrane-compromised cells in the untreated control (d), BioTe-treated (e), and tellurite-treated (f) groups. (g–i) SYTO 9 staining shows all cells in untreated (g), BioTe-treated (h), and tellurite-treated (i) samples. (j–l) Bright-field images of the corresponding samples. Scale bars for fluorescence and bright-field images: 10 μm. (m) β-Galactosidase activity in culture supernatants of E. coli BL21(DE3) overexpressing lacZ , treated with BioTe (3× MIC), tellurite (3× MIC), or left untreated for 1 hour. Samples were analyzed via SEM, fluorescence staining, and β-galactosidase activity measurement. Data represent mean ± SD ( n = 3). Asterisks indicate statistically significant differences ( p < 0.05).

    Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

    Techniques: Membrane, Staining, Control, Fluorescence, Activity Assay

    Antibacterial and antibiofilm activity of Te and Te–SO NNs against E. coli . (a) Colony count variation and (b) antibacterial efficacy at different concentrations of Te and Te–SO NNs. (c) Fluorescence microscopy images of E. coli stained with SYTO 9 (live cells, green) and PI (membrane-compromised cells, red) following treatment with Te and Te–SO NNs (100 μg mL −1 ). SEM images depicting morphological changes in (d) planktonic E. coli cells and (e) E. coli biofilms after exposure to Te or Te–SO NNs (100 μg mL −1 ).

    Journal: RSC Advances

    Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

    doi: 10.1039/d5ra02635k

    Figure Lengend Snippet: Antibacterial and antibiofilm activity of Te and Te–SO NNs against E. coli . (a) Colony count variation and (b) antibacterial efficacy at different concentrations of Te and Te–SO NNs. (c) Fluorescence microscopy images of E. coli stained with SYTO 9 (live cells, green) and PI (membrane-compromised cells, red) following treatment with Te and Te–SO NNs (100 μg mL −1 ). SEM images depicting morphological changes in (d) planktonic E. coli cells and (e) E. coli biofilms after exposure to Te or Te–SO NNs (100 μg mL −1 ).

    Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

    Techniques: Activity Assay, Fluorescence, Microscopy, Staining, Membrane

    In vivo therapeutic efficacy of Te NNs/PVA hydrogel on wounds co-infected with S. aureus and E. coli . (a) Representative images of wound healing progression in rats treated with PBS, PVA hydrogel, and Te NNs/PVA hydrogel at days 0, 4, 7, 10, and 14 (scale bar: 1 cm). (b) Bacterial culture images obtained from skin tissue samples across different treatment groups over the same period. (c) Quantitative analysis of wound healing rates from day 4 to day 14. (d) Residual bacterial counts at wound sites for each treatment group. Data are presented as mean ± standard deviation ( n = 5 per group); * p < 0.05, ** p < 0.01, compared to the Te NNs/PVA group.

    Journal: RSC Advances

    Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

    doi: 10.1039/d5ra02635k

    Figure Lengend Snippet: In vivo therapeutic efficacy of Te NNs/PVA hydrogel on wounds co-infected with S. aureus and E. coli . (a) Representative images of wound healing progression in rats treated with PBS, PVA hydrogel, and Te NNs/PVA hydrogel at days 0, 4, 7, 10, and 14 (scale bar: 1 cm). (b) Bacterial culture images obtained from skin tissue samples across different treatment groups over the same period. (c) Quantitative analysis of wound healing rates from day 4 to day 14. (d) Residual bacterial counts at wound sites for each treatment group. Data are presented as mean ± standard deviation ( n = 5 per group); * p < 0.05, ** p < 0.01, compared to the Te NNs/PVA group.

    Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

    Techniques: In Vivo, Drug discovery, Infection, Standard Deviation

    Histological analysis of skin tissue from S. aureus and E. coli co-infected wounds following treatment with different dressings. (a) Hematoxylin and eosin (H&E) stained images and (b) Masson's trichrome stained images, illustrating the extent of bacterial infection and tissue regeneration after 7 and 14 days of treatment. Scale bar: 100 μm. Yellow arrows indicate neutrophils, while blue arrows highlight fibroblasts.

    Journal: RSC Advances

    Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

    doi: 10.1039/d5ra02635k

    Figure Lengend Snippet: Histological analysis of skin tissue from S. aureus and E. coli co-infected wounds following treatment with different dressings. (a) Hematoxylin and eosin (H&E) stained images and (b) Masson's trichrome stained images, illustrating the extent of bacterial infection and tissue regeneration after 7 and 14 days of treatment. Scale bar: 100 μm. Yellow arrows indicate neutrophils, while blue arrows highlight fibroblasts.

    Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

    Techniques: Infection, Staining